Reverse Transcription
Reverse Transcription is the process in cells by which an enzyme makes a copy of DNA from RNA. The enzyme that makes the DNA copy is called reverse transcriptase. The reaction involve three steps, first primer annealing, followed by DNA polymerisation and finally enzyme deactivation. HighQu offer a complete range of reverse transcriptases, cDNA synthesis kits and RNAse inhibitors.
Reverse Transcriptase
HighScriber™ - a blend of Reverse Transcriptase and RNase Inhibitor for an efficient cDNA synthesis
Thermostable Reverse Transcriptase blended with Ribonuclease Inhibitor which achieves high yields of full lengths transcripts up to 12-15 kb, cDNA synthesis from complex templates at up to 55°C and high sensitivity detection from 1 pg of total RNA template.
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cDNA Synthesis Kits
qScriber™ cDNA Synthesis Kit: Fast and reliable cDNA synthesis for gene expression analysis, thermostable HighScriber™ Reverse Transcriptase blended with Ribonuclease Inhibitor for efficient cDNA synthesis.
Optimized reaction mix with oligo (dT) and random primers for unbiased representation of mRNA ends.
cDNA synthesis from complex templates at up to 55°C.
High sensitivity detection from 1 pg of total RNA template.
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RNAse Inhibititors
SecurRIN™ Advanced RNase Inhibitor: Robust RNA protection during sample preparation. Efficient RNAse inhibition even at higher temperatures up to 60°C. RNA protection from hydrolysis by RNases A, B and C. Economical & stable for weeks at ambient temperature. SecurRIN™ is very robust and works well at 5.5 to 9.0 pH, with 0.5 - 1 mM DTT.
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